Cloning and Expression of Recombinant Tick-Borne Encephalitis Virus-like Particles in Pichia pastoris.

ABSTRACT:

Objectives

The purpose of this study was to verify the feasibility of using the glyceraldehyde-3-phosphate dehydrogenase (GAP) promotor based Pichia pastoris expression system to produce tick-borne encephalitis virus (TBEV) virus-like particles (VLPs).

Methods

The complementary DNA encoding the TBEV prM signal peptide, prM, and E proteins of TBEV Korean strain (KrM 93) was cloned into the plasmid vector pGAPZɑA, then integrated into the genome of P. pastoris, under the control of the GAP promoter. Expression of TBEV VLPs was determined by Western blotting using monoclonal antibody against TBEV envelope (E) protein.

Results

Recombinant TBEV VLPs consisting of prM and E protein were successfully expressed using the GAP promoter-based P. pastoris expression system. The r

SUBMITTER: Yun SM 

PROVIDER: S-EPMC4225587 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature