Integrating multiple 'omics' analyses identifies serological protein biomarkers for preeclampsia.

ABSTRACT:

Background

Preeclampsia (PE) is a pregnancy-related vascular disorder which is the leading cause of maternal morbidity and mortality. We sought to identify novel serological protein markers to diagnose PE with a multi-'omics' based discovery approach.

Methods

Seven previous placental expression studies were combined for a multiplex analysis, and in parallel, two-dimensional gel electrophoresis was performed to compare serum proteomes in PE and control subjects. The combined biomarker candidates were validated with available ELISA assays using gestational age-matched PE (n=32) and control (n=32) samples. With the validated biomarkers, a genetic algorithm was then used to construct and optimize biomarker panels in PE assessment.

Results

In addition to the previously ide

SUBMITTER: Liu LY 

PROVIDER: S-EPMC4226208 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature