Using the epigenetic field defect to detect prostate cancer in biopsy negative patients.

ABSTRACT:

Purpose

We determined whether a novel combination of field defect DNA methylation markers could predict the presence of prostate cancer using histologically normal transrectal ultrasound guided biopsy cores.

Materials and methods

Methylation was assessed using quantitative Pyrosequencing® in a training set consisting of 65 nontumor and tumor associated prostate tissues from University of Wisconsin. A multiplex model was generated using multivariate logistic regression and externally validated in blinded fashion in a set of 47 nontumor and tumor associated biopsy specimens from University of Washington.

Results

We observed robust methylation differences in all genes at all CpGs assayed (p <0.0001). Regression models incorporating individual genes (EVX1, CAV1 and FGF1)

SUBMITTER: Truong M 

PROVIDER: S-EPMC4226217 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature