Evaluation of exogenous siRNA addition as a metabolic engineering tool for modifying biopharmaceuticals.

ABSTRACT: Traditional metabolic engineering approaches, including homologous recombination, zinc-finger nucleases, and short hairpin RNA, have previously been used to generate biologics with specific characteristics that improve efficacy, potency, and safety. An alternative approach is to exogenously add soluble small interfering RNA (siRNA) duplexes, formulated with a cationic lipid, directly to cells grown in shake flasks or bioreactors. This approach has the following potential advantages: no cell line development required, ability to tailor mRNA silencing by adjusting siRNA concentration, simultaneous silencing of multiple target genes, and potential temporal control of down regulation of target gene expression. In this study, we demonstrate proof of concept of the siRNA feeding approach as a me

SUBMITTER: Tummala S 

PROVIDER: S-EPMC4226405 | biostudies-literature | 2013 Mar-Apr

REPOSITORIES: biostudies-literature