Spinophilin expression determines cellular growth, cancer stemness and 5-flourouracil resistance in colorectal cancer.

ABSTRACT: The putative tumor suppressor gene spinophilin has been involved in cancer progression in several types of cancer. In this study, we explored the prognostic value of spinophilin expression in 162 colon adenocarcinoma patients. In addition, we generated stably expressing spinophilin-directed shRNA CRC cell lines and studied the influence of spinophilin expression on cellular phenotypes and molecular interactions. We independently confirmed that low spinophilin expression levels are associated with poor prognosis in CRC patients (p = 0.038). A reduction of spinophilin levels in p53 wild-type HCT116 and p53-mutated Caco-2 cells led to increased cellular growth rates and anchorage-independent growth (p<0.05). At molecular level, reduced spinophilin levels increased the expression of the transc

SUBMITTER: Ress AL 

PROVIDER: S-EPMC4226699 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature