Hypercholesterolemia inhibits re-endothelialization of arterial injuries by TRPC channel activation.

ABSTRACT:

Objective

After arterial injury, endothelial cell (EC) migration is essential for healing, but lipid oxidation products activate TRPC6 and TRPC5 ion channels, leading to increased intracellular calcium and inhibition of EC migration in vitro. The objective of this study was to further evaluate the role of TRPC channels in EC migration in vitro and to validate in vitro findings in an in vivo model.

Methods

Mouse aortic ECs were cultured, and the effect of lysophosphatidylcholine, the major lysophospholipid in oxidized low-density lipoprotein, on migration was assessed in a razor-scrape assay. EC healing after a carotid injury with electrocautery was evaluated in wild-type (WT), TRPC6(-/-), and TRPC5(-/-) mice receiving either a chow or high-cholesterol (HC) diet.

Results</

SUBMITTER: Rosenbaum MA 

PROVIDER: S-EPMC4226809 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature