MXD3 regulation of DAOY cell proliferation dictated by time course of activation.

ABSTRACT:

Background

MXD3 is a basic-helix-loop-helix-leucine-zipper transcription factor involved in cellular proliferation. In previous studies we demonstrated that knock-down of MXD3 in the human medulloblastoma cell line DAOY resulted in decreased proliferation. Surprisingly, overexpression of MXD3 in DAOY cells also decreased proliferation and increased cell death, suggesting that persistent expression of MXD3 triggers an apoptotic response, perhaps as a fail-safe mechanism. To investigate this apparent paradox in detail we developed a tamoxifen inducible system to analyze the temporal effects of MXD3 in the proliferation and transcriptional response of DAOY cells upon acute induction compared with long-term expression of MXD3.

Results

We find that acute induction of MXD3 initial

SUBMITTER: Ngo T 

PROVIDER: S-EPMC4226952 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature