Production of phytotoxic cationic α-helical antimicrobial peptides in plant cells using inducible promoters.

ABSTRACT: Synthetic linear antimicrobial peptides with cationic α-helical structures, such as BP100, have potent and specific activities against economically important plant pathogenic bacteria. They are also recognized as valuable therapeutics and preservatives. However, highly active BP100 derivatives are often phytotoxic when expressed at high levels as recombinant peptides in plants. Here we demonstrate that production of recombinant phytotoxic peptides in transgenic plants is possible by strictly limiting transgene expression to certain tissues and conditions, and specifically that minimization of this expression during transformation and regeneration of transgenic plants is essential to obtain viable plant biofactories. On the basis of whole-genome transcriptomic data available online, we iden

SUBMITTER: Company N 

PROVIDER: S-EPMC4227650 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature