Hypoxic conditioned medium from rat cerebral cortical cells enhances the proliferation and differentiation of neural stem cells mainly through PI3-K/Akt pathways.

ABSTRACT:

Purpose

To investigate the effects of hypoxic conditioned media from rat cerebral cortical cells on the proliferation and differentiation of neural stem cells (NSCs) in vitro, and to study the roles of PI3-K/Akt and JNK signal transduction pathways in these processes.

Methods

Cerebral cortical cells from neonatal Sprague-Dawley rat were cultured under hypoxic and normoxic conditions; the supernatant was collected and named 'hypoxic conditioned medium' (HCM) and 'normoxic conditioned medium' (NCM), respectively. We detected the protein levels (by ELISA) of VEGF and BDNF in the conditioned media and mRNA levels (by RT-PCR) in cerebral cortical cells. The proliferation (number and size of neurospheres) and differentiation (proportion of neurons and astrocytes over total cells)

SUBMITTER: Cai M 

PROVIDER: S-EPMC4227679 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature