Identification of novel knockout targets for improving terpenoids biosynthesis in Saccharomyces cerevisiae.

ABSTRACT: Many terpenoids have important pharmacological activity and commercial value; however, application of these terpenoids is often limited by problems associated with the production of sufficient amounts of these molecules. The use of Saccharomyces cerevisiae (S. cerevisiae) for the production of heterologous terpenoids has achieved some success. The objective of this study was to identify S. cerevisiae knockout targets for improving the synthesis of heterologous terpeniods. On the basis of computational analysis of the S. cerevisiae metabolic network, we identified the knockout sites with the potential to promote terpenoid production and the corresponding single mutant was constructed by molecular manipulations. The growth rates of these strains were measured and the results indicated that t

SUBMITTER: Sun Z 

PROVIDER: S-EPMC4227703 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature