Expression and purification of the antimicrobial peptide GSL1 in bacteria for raising antibodies.

ABSTRACT:

Background

The Gibberellin Stimulated-Like (GSL) or Snakin peptides from higher plants are cysteine-rich, with broad spectrum activity against a range of bacterial and fungal pathogens. To detect GSL peptides in applications such as western blot analysis and enzyme-linked immunosorbent assays (ELISA), specific antibodies that recognise GSL peptides are required. However, the intrinsic antimicrobial activity of these peptides is likely to prevent their expression alone in bacterial or yeast expression systems for subsequent antibody production in animal hosts.

Results

To overcome this issue we developed an Escherichia coli expression strategy based on the expression of the GSL1 peptide as a His-tagged thioredoxin fusion protein. The DNA sequence for the mature GSL1 peptide fr

SUBMITTER: Meiyalaghan S 

PROVIDER: S-EPMC4228058 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature