Cytosolic galectin-7 impairs p53 functions and induces chemoresistance in breast cancer cells.

ABSTRACT:

Background

Resistance to apoptosis induced by anti-cancer drugs is a major obstacle for the treatment of aggressive forms of breast cancer. Galectin-7 (gal-7) was recently shown to be specifically expressed in basal-like but not in luminal subtypes of human breast cancer.

Methods

We generated a mutant form of gal-7 (R74S). Arginine 74 is the structural equivalent of arginine 186 found in human galectin-3. Mutation R186S was previously shown to abolish the biological function of galectin-3.

Results

Mutation of arginine 74 induced only limited and local changes to the gal-7 fold. Recombinant forms of R74S and wtgal-7 were also equally effective at forming dimers in solution. Analysis of the thermodynamic parameters by isothermal titration calorimetry (ITC) indicated, ho

SUBMITTER: Grosset AA 

PROVIDER: S-EPMC4228062 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature