Ubiquitin-proteasome dependent degradation of GABAAα1 in autism spectrum disorder.

ABSTRACT:

Background

Although the neurobiological basis of autism spectrum disorder (ASD) is not fully understood, recent studies have indicated the potential role of GABAA receptors in the pathophysiology of ASD. GABAA receptors play a crucial role in various neurodevelopmental processes and adult neuroplasticity. However, the mechanism(s) of regulation of GABAA receptors in ASD remains poorly understood.

Methods

Postmortem middle frontal gyrus tissues (13 ASD and 13 control subjects) were used. In vitro studies were performed in primary cortical neurons at days in vitro (DIV) 14. The protein levels were examined by western blotting. Immunofluorescence studies were employed for cellular localization. The gene expression was determined by RT-PCR array and qRT-PCR.

Results

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SUBMITTER: Crider A 

PROVIDER: S-EPMC4228821 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature