Extracellular vesicles: a platform for the structure determination of membrane proteins by Cryo-EM.

ABSTRACT: Membrane protein-enriched extracellular vesicles (MPEEVs) provide a platform for studying intact membrane proteins natively anchored with the correct topology in genuine biological membranes. This approach circumvents the need to conduct tedious detergent screens for solubilization, purification, and reconstitution required in classical membrane protein studies. We have applied this method to three integral type I membrane proteins, namely the Caenorhabditis elegans cell-cell fusion proteins AFF-1 and EFF-1 and the glycoprotein B (gB) from Herpes simplex virus type 1 (HSV1). Electron cryotomography followed by subvolume averaging allowed the 3D reconstruction of EFF-1 and HSV1 gB in the membrane as well as an analysis of the spatial distribution and interprotein interactions on the membran

SUBMITTER: Zeev-Ben-Mordehai T 

PROVIDER: S-EPMC4229021 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature