Functional heterologous protein expression by genetically engineered probiotic yeast Saccharomyces boulardii.

ABSTRACT: Recent studies have suggested the potential of probiotic organisms to be adapted for the synthesis and delivery of oral therapeutics. The probiotic yeast Saccharomyces boulardii would be especially well suited for this purpose due to its ability, in contrast to probiotic prokaryotes, to perform eukaryotic post translational modifications. This probiotic yeast thus has the potential to express a broad array of therapeutic proteins. Currently, however, use of wild type (WT) S. boulardii relies on antibiotic resistance for the selection of transformed yeast. Here we report the creation of auxotrophic mutant strains of S. boulardii that can be selected without antibiotics and demonstrate that these yeast can express functional recombinant protein even when recovered from gastrointestinal immun

SUBMITTER: Hudson LE 

PROVIDER: S-EPMC4229219 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature