Use of independent component analysis to improve signal-to-noise ratio in multi-probe fluorescence microscopy.

ABSTRACT: In conventional multi-probe fluorescence microscopy, narrow bandwidth filters on detectors are used to avoid bleed-through artefacts between probes. The limited bandwidth reduces the signal-to-noise ratio of the detection, often severely compromising one or more channels. Herein, we describe a process of using independent component analysis to discriminate the position of different probes using only a dichroic mirror to differentiate the signals directed to the detectors. Independent component analysis was particularly effective in samples where the spatial overlap between the probes is minimal, a very common case in cellular microscopy. This imaging scheme collects nearly all of the emitted light, significantly improving the image signal-to-noise ratio. In this study, we focused on the de

SUBMITTER: Dao L 

PROVIDER: S-EPMC4229364 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature