Serum microRNA panel as biomarkers for early diagnosis of colorectal adenocarcinoma.

ABSTRACT:

Background

Currently, none of the available colorectal adenocarcinoma (CAC) testing has been established as a well-accepted diagnosis tool, particularly for the early stage of CAC. The recent discovery of serum microRNA (miRNA) profile has provided a new auxiliary approach for tumour diagnosis. Our study is involved in the global analysis of serum miRNAs during the normal-colorectal adenoma (CA)-CAC sequence.

Methods

Serum samples were collected from 307 CAC patients, 164 CA patients and 226 healthy controls. Differentially expressed serum miRNAs were screened with Miseq sequencing followed by the reverse transcription PCR (RT-qPCR) validation. The miRNA panel was developed with a logistic regression model and validated using an independent cohort. The miRNA levels in CAC pa

SUBMITTER: Zheng G 

PROVIDER: S-EPMC4229633 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature