Shed Syndecan-1 is involved in chemotherapy resistance via the EGFR pathway in colorectal cancer.

ABSTRACT:

Background

Syndecan-1 (Sdc-1) shedding induced by matrix metalloproteinase-7 (MMP-7) and additional proteases has an important role in cancer development. However, the impact of Sdc-1 shedding on chemotherapeutic resistance has not been reported.

Methods

We examined Sdc-1 shedding in colorectal cancer by enzyme-linked immunosorbent assay (ELISA), Dot blot, reverse transcription-PCR (RT-PCR), immunohistochemistry and so on, its impact on chemotherapeutic sensitivity by collagen gel droplet embedded culture-drug sensitivity test (CD-DST) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), and potential mechanisms of action by Dot blot, western blot and immunofluorescence.

Results

Sdc-1 shedding was increased in colorectal cancer patients, Sdc-1 seru

SUBMITTER: Wang X 

PROVIDER: S-EPMC4229635 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature