Characterization of the binding interaction between the oncoprotein gankyrin and a grafted S6 ATPase.

ABSTRACT: A complex with the C-terminal portion of the proteosomal subunit S6 ATPase is the only available structure of a protein-protein interaction involving the oncoprotein gankyrin. However, difficulties associated with recombinant expression of S6 ATPase alone, or truncations thereof, have limited our understanding of this assembly. We replaced the C-terminal portion of FtsH from Escherichia coli with the structurally homologous C-terminal portion of S6 ATPase and used this grafted protein to characterize the gankyrin-S6 ATPase binding interaction by isothermal titration calorimetry.

SUBMITTER: Chapman AM 

PROVIDER: S-EPMC4230329 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature