Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope.

ABSTRACT: Single-particle tracking has been applied to study chromatin motion in live cells, revealing a wealth of dynamical behavior of the genomic material once believed to be relatively static throughout most of the cell cycle. Here we used the dual-color three-dimensional (3D) double-helix point spread function microscope to study the correlations of movement between two fluorescently labeled gene loci on either the same or different budding yeast chromosomes. We performed fast (10 Hz) 3D tracking of the two copies of the GAL locus in diploid cells in both activating and repressive conditions. As controls, we tracked pairs of loci along the same chromosome at various separations, as well as transcriptionally orthogonal genes on different chromosomes. We found that under repressive conditions, th

SUBMITTER: Backlund MP 

PROVIDER: S-EPMC4230621 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature