The RNA-binding protein Staufen1 impairs myogenic differentiation via a c-myc-dependent mechanism.

ABSTRACT: Recent work has shown that Staufen1 plays key roles in skeletal muscle, yet little is known about its pattern of expression during embryonic and postnatal development. Here we first show that Staufen1 levels are abundant in mouse embryonic muscles and that its expression decreases thereafter, reaching low levels in mature muscles. A similar pattern of expression is seen as cultured myoblasts differentiate into myotubes. Muscle degeneration/regeneration experiments revealed that Staufen1 increases after cardiotoxin injection before returning to the low levels seen in mature muscles. We next prevented the decrease in Staufen1 during differentiation by generating stable C2C12 muscle cell lines overexpressing Staufen1. Cells overexpressing Staufen1 differentiated poorly, as evidenced by reduct

SUBMITTER: Ravel-Chapuis A 

PROVIDER: S-EPMC4230783 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature