Selection of reference genes for quantitative real-time PCR normalization in Panax ginseng at different stages of growth and in different organs.

ABSTRACT: Quantitative real-time reverse transcription PCR (qRT-PCR) has become a widely used method for gene expression analysis; however, its data interpretation largely depends on the stability of reference genes. The transcriptomics of Panax ginseng, one of the most popular and traditional ingredients used in Chinese medicines, is increasingly being studied. Furthermore, it is vital to establish a series of reliable reference genes when qRT-PCR is used to assess the gene expression profile of ginseng. In this study, we screened out candidate reference genes for ginseng using gene expression data generated by a high-throughput sequencing platform. Based on the statistical tests, 20 reference genes (10 traditional housekeeping genes and 10 novel genes) were selected. These genes were tested for th

SUBMITTER: Liu J 

PROVIDER: S-EPMC4230945 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature