Protein thermal stability enhancement by designing salt bridges: a combined computational and experimental study.

ABSTRACT: Protein thermal stability is an important factor considered in medical and industrial applications. Many structural characteristics related to protein thermal stability have been elucidated, and increasing salt bridges is considered as one of the most efficient strategies to increase protein thermal stability. However, the accurate simulation of salt bridges remains difficult. In this study, a novel method for salt-bridge design was proposed based on the statistical analysis of 10,556 surface salt bridges on 6,493 X-ray protein structures. These salt bridges were first categorized based on pairing residues, secondary structure locations, and Cα-Cα distances. Pairing preferences generalized from statistical analysis were used to construct a salt-bridge pair index and utilized in a weighted

SUBMITTER: Lee CW 

PROVIDER: S-EPMC4231051 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature