Genome-wide microarray expression and genomic alterations by array-CGH analysis in neuroblastoma stem-like cells.

ABSTRACT: Neuroblastoma has a very diverse clinical behaviour: from spontaneous regression to a very aggressive malignant progression and resistance to chemotherapy. This heterogeneous clinical behaviour might be due to the existence of Cancer Stem Cells (CSC), a subpopulation within the tumor with stem-like cell properties: a significant proliferation capacity, a unique self-renewal capacity, and therefore, a higher ability to form new tumors. We enriched the CSC-like cell population content of two commercial neuroblastoma cell lines by the use of conditioned cell culture media for neurospheres, and compared genomic gains and losses and genome expression by array-CGH and microarray analysis, respectively (in CSC-like versus standard tumor cells culture). Despite the array-CGH did not show significa

SUBMITTER: Ordonez R 

PROVIDER: S-EPMC4231109 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature