Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.

ABSTRACT: Over the years, Chinese hamster ovary (CHO) cells have emerged as the major host for expressing biotherapeutic proteins. Traditional methods to generate high-producer cell lines rely on random integration(s) of the gene of interest but have thereby left the identification of bottlenecks as a challenging task. For comparison of different producer cell lines derived from various transfections, a system that provides control over transgene expression behavior is highly needed. This motivated us to develop a novel "DUKX-B11 F3/F" cell line to target different single-chain antibody fragments into the same chromosomal target site by recombinase-mediated cassette exchange (RMCE) using the flippase (FLP)/FLP recognition target (FRT) system. The RMCE-competent cell line contains a gfp reporter fuse

SUBMITTER: Mayrhofer P 

PROVIDER: S-EPMC4231286 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature