Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae.

ABSTRACT:

Background

Manipulations in Saccharomyces cerevisiae classically depend on use of auxotrophy selection markers. There are several disadvantages to this in a microbial cell factory setting: (1) auxotrophies must first be engineered in prototrophic strains, and many industrial strains are polyploid/aneuploid prototrophs (2) available strain auxotrophies must be paired with available repair plasmids (3) remaining auxotrophies must be repaired prior to development of industrial bioprocesses. Use of dominant antibiotic resistance markers can circumvent these problems. However, there are relatively few yeast antibiotic resistance marker vectors available; furthermore, available vectors contain only one expression cassette, and it is often desirable to introduce more than one gene at a ti

SUBMITTER: Vickers CE 

PROVIDER: S-EPMC4231455 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature