Shape matters: size-exclusion HPLC for the study of nucleic acid structural polymorphism.

ABSTRACT: In recent years, an increasing number of reports have been focused on the structure and biological role of non-canonical nucleic acid secondary structures. Many of these studies involve the use of oligonucleotides that can often adopt a variety of structures depending on the experimental conditions, and hence change the outcome of an assay. The knowledge of the structure(s) formed by oligonucleotides is thus critical to correctly interpret the results, and gain insight into the biological role of these particular sequences. Herein we demonstrate that size-exclusion HPLC (SE-HPLC) is a simple yet surprisingly powerful tool to quickly and effortlessly assess the secondary structure(s) formed by oligonucleotides. For the first time, an extensive calibration and validation of the use of SE-HPL

SUBMITTER: Largy E 

PROVIDER: S-EPMC4231728 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature