Large-scale mapping of sequence-function relations in small regulatory RNAs reveals plasticity and modularity.

ABSTRACT: Two decades into the genomics era the question of mapping sequence to function has evolved from identifying functional elements to characterizing their quantitative properties including, in particular, their specificity and efficiency. Here, we use a large-scale approach to establish a quantitative map between the sequence of a bacterial regulatory RNA and its efficiency in modulating the expression of its targets. Our approach generalizes the sort-seq method, introduced recently to analyze promoter sequences, in order to accurately quantify the efficiency of a large library of sequence variants. We focus on two small RNAs (sRNAs) in E. coli, DsrA and RyhB, and their regulation of both repressed and activated targets. In addition to precisely identifying functional elements in the sRNAs, o

SUBMITTER: Peterman N 

PROVIDER: S-EPMC4231740 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature