Quantitative effect of target translation on small RNA efficacy reveals a novel mode of interaction.

ABSTRACT: Small regulatory RNAs (sRNAs) in bacteria regulate many important cellular activities under normal conditions and in response to stress. Many sRNAs bind to the mRNA targets at or near the 5' untranslated region (UTR) resulting in translation inhibition and accelerated degradation. Often the sRNA-binding site is adjacent to or overlapping with the ribosomal binding site (RBS), suggesting a possible interplay between sRNA and ribosome binding. Here we combine quantitative experiments with mathematical modeling to reveal novel features of the interaction between small RNAs and the translation machinery at the 5'UTR of a target mRNA. By measuring the response of a library of reporter targets with varied RBSs, we find that increasing translation rate can lead to increased repression. Quantitati

SUBMITTER: Lavi-Itzkovitz A 

PROVIDER: S-EPMC4231754 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature