Local burn injury impairs epithelial permeability and antimicrobial peptide barrier function in distal unburned skin.

ABSTRACT:

Objectives

Our objective was to characterize the mechanisms by which local burn injury compromises epithelial barrier function in burn margin, containing the elements necessary for healing of the burn site, and in distal unburned skin, which serves as potential donor tissue.

Design

Experimental mouse scald burn injury.

Setting

University Research Laboratory.

Subjects

C57/Bl6 Male mice, 8-12 weeks old.

Interventions

To confirm that dehydration was not contributing to our observed barrier defects, in some experiments mice received 1 mL of saline fluid immediately after burn, while a subgroup received an additional 0.5 mL at 4 hours and 1 mL at 24 hours following burn. We then assessed skin pH and transepidermal water loss every 12 hours on the burn wounds

SUBMITTER: Plichta JK 

PROVIDER: S-EPMC4231880 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature