Role of the endothelin axis in astrocyte- and endothelial cell-mediated chemoprotection of cancer cells.

ABSTRACT:

Background

Recent evidence suggests that astrocytes protect cancer cells from chemotherapy by stimulating upregulation of anti-apoptotic genes in those cells. We investigated the possibility that activation of the endothelin axis orchestrates survival gene expression and chemoprotection in MDA-MB-231 breast cancer cells and H226 lung cancer cells.

Methods

Cancer cells, murine astrocytes, and murine fibroblasts were grown in isolation, and expression of endothelin (ET) peptides and ET receptors (ETAR and ETBR) compared with expression on cancer cells and astrocytes (or cancer cells and fibroblasts) that were co-incubated for 48 hours. Type-specific endothelin receptor antagonists were used to evaluate the contribution of ETAR and ETBR to astrocyte-induced activation of the pr

SUBMITTER: Kim SW 

PROVIDER: S-EPMC4232084 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature