Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells.

ABSTRACT:

Background

The objective of the present study was to identify human epididymis protein 4 (HE4) interacting proteins and explore the mechanisms underlying their effect on ovarian cancer cell invasion and metastasis.

Methods

HE4 interacting proteins were identified by mass spectrometry and validated by co-immunoprecipitation and pull-down assays. The scratch test, the Transwell assay and animal experiments were used to assess the invasive and metastatic abilities of ovarian cancer cells before and after transfection and HE4 protein treatment. HE4 and annexin II protein expression in epithelial ovarian tissues was detected by immunohistochemistry, and the relation between their expression levels was examined.

Results

Annexin II was identified as an HE4 interacting protei

SUBMITTER: Zhuang H 

PROVIDER: S-EPMC4232681 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature