Whole-genome enrichment and sequencing of Chlamydia trachomatis directly from clinical samples.

ABSTRACT:

Background

Chlamydia trachomatis is a pathogen of worldwide importance, causing more than 100 million cases of sexually transmitted infections annually. Whole-genome sequencing is a powerful high resolution tool that can be used to generate accurate data on bacterial population structure, phylogeography and mutations associated with antimicrobial resistance. The objective of this study was to perform whole-genome enrichment and sequencing of C. trachomatis directly from clinical samples.

Methods

C. trachomatis positive samples comprising seven vaginal swabs and three urine samples were sequenced without prior in vitro culture in addition to nine cultured C. trachomatis samples, representing different serovars. A custom capture RNA bait set, that captures all known diversity

SUBMITTER: Christiansen MT 

PROVIDER: S-EPMC4233057 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature