The hepatitis C virus core protein can modulate RNA-dependent RNA synthesis by the 2a polymerase.

ABSTRACT: RNA replication enzymes are multi-subunit protein complexes whose activity can be modulated by other viral and cellular factors. For genotype 1b Hepatitis C virus (HCV), the RNA-dependent RNA polymerase (RdRp) subunit of the replicase, NS5B, has been reported to interact with the HCV Core protein to decrease RNA synthesis (Kang et al., 2009). Here we used a cell-based assay for RNA synthesis to examine the Core-NS5B interaction of genotype 2a HCV. Unlike the 1b NS5B, the activity of the 2a NS5B was stimulated by the Core protein. Using the bimolecular fluorescence complementation assay, the 2a Core co-localized with 2a NS5B when they were transiently expressed in cells. The two proteins can form a co-immunoprecipitable complex. Deletion analysis showed that the N-terminal 75 residues of 2a

SUBMITTER: Wen Y 

PROVIDER: S-EPMC4233142 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature