Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data.

ABSTRACT: The analysis of single molecule imaging experiments is complicated by the stochastic nature of single molecule events, by instrument noise and by the limited information which can be gathered about any individual molecule observed. Consequently, it is important to cross check experimental results using a model simulating single molecule dynamics (e.g. movements and binding events) in a virtual cell-like environment. The output of such a model should match the real data format allowing researchers to compare simulated results with the real experiments. The proposed model exploits the advantages of 'object-oriented' computing. First of all, the ability to create and manipulate a number of classes, each containing an arbitrary number of single molecule objects. These classes may include objec

SUBMITTER: Mashanov GI 

PROVIDER: S-EPMC4233692 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature