Transcriptome-wide analysis of messenger RNA decay in normal and osteoarthritic human articular chondrocytes.

ABSTRACT:

Objective

Messenger RNA (mRNA) decay rates control not only gene expression levels, but also responsiveness to altered transcriptional input. We undertook this study to examine transcriptome-wide posttranscriptional regulation in both normal and osteoarthritic (OA) human articular chondrocytes.

Methods

Human articular chondrocytes were isolated from normal or OA tissue. Equine articular chondrocytes were isolated from young or old horses at a commercial abattoir. RNA decay was measured across the transcriptome in human cells by microarray analysis following an actinomycin D chase. Messenger RNA levels in samples were confirmed using quantitative reverse transcription-polymerase chain reaction.

Results

Examination of total mRNA expression levels demonstrated significan

SUBMITTER: Tew SR 

PROVIDER: S-EPMC4233952 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature