Rapid optical determination of β-lactamase and antibiotic activity.

ABSTRACT:

Background

The absence of rapid tests evaluating antibiotic susceptibility results in the empirical prescription of antibiotics. This can lead to treatment failures due to escalating antibiotic resistance, and also furthers the emergence of drug-resistant bacteria. This study reports a rapid optical method to detect β-lactamase and thereby assess activity of β-lactam antibiotics, which could provide an approach for targeted prescription of antibiotics. The methodology is centred on a fluorescence quenching based probe (β-LEAF--β-Lactamase Enzyme Activated Fluorophore) that mimics the structure of β-lactam antibiotics.

Results

The β-LEAF assay was performed for rapid determination of β-lactamase production and activity of β-lactam antibiotic (cefazolin) on a panel of Staphylo

SUBMITTER: Khan S 

PROVIDER: S-EPMC4234275 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature