Microbiota diversity and gene expression dynamics in human oral biofilms.

ABSTRACT:

Background

Micro-organisms inhabiting teeth surfaces grow on biofilms where a specific and complex succession of bacteria has been described by co-aggregation tests and DNA-based studies. Although the composition of oral biofilms is well established, the active portion of the bacterial community and the patterns of gene expression in vivo have not been studied.

Results

Using RNA-sequencing technologies, we present the first metatranscriptomic study of human dental plaque, performed by two different approaches: (1) A short-reads, high-coverage approach by Illumina sequencing to characterize the gene activity repertoire of the microbial community during biofilm development; (2) A long-reads, lower-coverage approach by pyrosequencing to determine the taxonomic identity of the a

SUBMITTER: Benitez-Paez A 

PROVIDER: S-EPMC4234424 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature