Primary porcine proximal tubular cells as an alternative to human primary renal cells in vitro: an initial characterization.

ABSTRACT:

Background

A good in vitro model should approximate an in vivo-like behavior as closely as possible in order to reflect most likely the in vivo situation. Regarding renal physiology of different species, humans are more closely related to pigs than to rodents, therefore primary porcine kidney cells (PKC) and their subsequent cell strain could be a valid alternative to primary human cells for renal in vitro toxicology. For this PKC must display inherent characteristics (e.g. structural organization) and functions (e.g. transepithelial transport) as observed under in vivo conditions within the respective part of the kidney.

Results

We carried out a comprehensive characterization of PKC and their subsequent cell strain, including morphology and growth as well as transporter exp

SUBMITTER: Heussner AH 

PROVIDER: S-EPMC4234457 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature