Role of Pnn in alternative splicing of a specific subset of lncRNAs of the corneal epithelium.

ABSTRACT:

Purpose

GG-H whole transcriptome array analysis suggested involvement of PININ (PNN) in the alternative splicing of multiple long non-coding RNAs (lncRNAs). To further investigate PNN's role in regulating the alternative splicing of lncRNAs in a corneal epithelial context, we performed detailed analyses for detecting and identifying alternatively spliced lncRNAs.

Methods

Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. Alternatively spliced lncRNAs were detected with in situ hybridization with variant-specific RNA probes on human cornea sections.

Results

Our analysis uncovered PNN's impact on the transcript

SUBMITTER: Joo JH 

PROVIDER: S-EPMC4235046 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature