Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification.

ABSTRACT: The microflora in environmental water consists of a high density and diversity of bacterial species that form the foundation of the water ecosystem. Because the majority of these species cannot be cultured in vitro, a different approach is needed to identify prokaryotes in environmental water. A novel DNA microarray was developed as a simplified detection protocol. Multiple DNA probes were designed against each of the 97,927 sequences in the DNA Data Bank of Japan and mounted on a glass chip in duplicate. Evaluation of the microarray was performed using the DNA extracted from one liter of environmental water samples collected from seven sites in Japan. The extracted DNA was uniformly amplified using whole genome amplification (WGA), labeled with Cy3-conjugated 16S rRNA specific primers and

SUBMITTER: Akama T 

PROVIDER: S-EPMC4235703 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature