Combination of environmental stress and localization of L-asparaginase in Arthrospira platensis for production improvement.

ABSTRACT: The diverse applications of L-asparaginase have led us to explore new sources of this enzyme. Arthrospira platensis has been scarcely reported as a new candidate for L-asparaginase production. In the present study, we localized L-asparaginase in A. platensis and enhanced its production. Enzyme localization was conducted by culturing cells in SOT medium and extracting the enzymes from different parts of the cell. The Taguchi method (factors studied: nitrogen, iron, sodium chloride, and temperature shock) using an L9 orthogonal array was designed for improving L-asparaginase production. The highest specific activity of L-asparaginase was found in subcellular, cytoplasmic extracts (0.166 ± 0.029 U/mg). Optimization data revealed that the highest production of L-asparaginase (0.275 ± 0.005 U)

SUBMITTER: Prihanto AA 

PROVIDER: S-EPMC4235887 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature