Anatomo-proteomic characterization of human basal ganglia: focus on striatum and globus pallidus.

ABSTRACT:

Background

The basal ganglia (BG) are a complex network of subcortical nuclei involved in the coordination and integration of the motor activity. Although these independent anatomical structures are functionally related, the proteome present in each isolated nucleus remains largely unexplored. In order to analyse the BG proteome in a large-scale format, we used a multi-dimensional fractionation approach which combines isolation of anatomically-defined nuclei, and protein/peptide chromatographic fractionation strategies coupled to mass spectrometry.

Results

Using this workflow, we have obtained a proteomic expression profile across striatum and globus pallidus structures among which 1681 proteins were located in caudate nucleus (CN), 1329 in putamen, 1419 in medial globus pal

SUBMITTER: Fernandez-Irigoyen J 

PROVIDER: S-EPMC4236423 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature