Development and characterization of low α-linolenic acid Brassica oleracea lines bearing a novel mutation in a 'class a' FATTY ACID DESATURASE 3 gene.

ABSTRACT:

Background

Traditional canola (Brassica napus L.; AACC, 2n=38) cultivars yield seed oil with a relatively high proportion of α-linolenic acid (ALA; C18:3cis∆9,12,15), which is desirable from a health perspective. Unfortunately, due to the instability of this fatty acid, elevated levels also result in oils that exhibit a short shelf life and problems associated with use at high temperatures. As a result, the development of cultivars bearing reduced amounts of ALA in their seeds is becoming a priority. To date, several low ALA B. napus cultivars (~2-3% ALA of total fatty acids) have been developed and molecular analyses have revealed that the low ALA phenotype of lines tested thus far is a result of mutations within two 'class b' FATTY ACID DESATURASE 3 (FAD3) genes. Since B. napus p

SUBMITTER: Singer SD 

PROVIDER: S-EPMC4236532 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature