Structural insight into the recognition of amino-acylated initiator tRNA by eIF5B in the 80S initiation complex.

ABSTRACT:

Background

From bacteria to eukarya, the specific recognition of the amino-acylated initiator tRNA by the universally conserved translational GTPase eIF5B/IF2 is one of the most central interactions in the process of translation initiation. However, the molecular details, particularly also in the context of ribosomal initiation complexes, are only partially understood.

Results

A reinterpretation of the 6.6 Å resolution cryo-electron microscopy (cryo-EM) structure of the eukaryal 80S initiation complex using the recently published crystal structure of eIF5B reveals that domain IV of eIF5B forms extensive interaction interfaces with the Met-tRNAi, which, in contrast to the previous model, directly involve the methionylated 3' CCA-end of the acceptor stem. These contacts are me

SUBMITTER: Kuhle B 

PROVIDER: S-EPMC4236685 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature