Molecular diagnostics on the toxigenic potential of Fusarium spp. plant pathogens.

ABSTRACT:

Aims

We propose and test an efficient and rapid protocol for the detection of toxigenic Fusarium isolates producing three main types of Fusarium-associated mycotoxins (fumonisins, trichothecenes and zearelanone).

Methods and results

The novel approach utilizes partially multiplexed markers based on genes essential for mycotoxin biosynthesis (fumonisin--fum6, fum8; trichothecenes--tri5, tri6; zearalenone, zea2) in Fusarium spp. The protocol has been verified by screening a collection of 96 isolates representing diverse species of filamentous fungi. Each Fusarium isolate was taxonomically identified through both molecular and morphological techniques. The results demonstrate a reliable detection of toxigenic potential for trichothecenes (sensitivity 100%, specificity 95%), zea

SUBMITTER: Dawidziuk A 

PROVIDER: S-EPMC4237539 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature