Genetic insulin resistance is a potent regulator of gene expression and proliferation in human iPS cells.

ABSTRACT: Insulin resistance is central to diabetes and metabolic syndrome. To define the consequences of genetic insulin resistance distinct from those secondary to cellular differentiation or in vivo regulation, we generated induced pluripotent stem cells (iPSCs) from individuals with insulin receptor mutations and age-appropriate control subjects and studied insulin signaling and gene expression compared with the fibroblasts from which they were derived. iPSCs from patients with genetic insulin resistance exhibited altered insulin signaling, paralleling that seen in the original fibroblasts. Insulin-stimulated expression of immediate early genes and proliferation were also potently reduced in insulin resistant iPSCs. Global gene expression analysis revealed marked differences in both insulin-resi

SUBMITTER: Iovino S 

PROVIDER: S-EPMC4238001 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature