Protein kinase C δ deficiency enhances megakaryopoiesis and recovery from thrombocytopenia.

ABSTRACT:

Objective

We previously determined that protein kinase C δ (PKCδ) regulates platelet function. However, the function of PKCδ in megakaryopoiesis is unknown.

Approach and results

Using PKCδ(-/-) and wild-type littermate mice, we found that deficiency of PKCδ caused an increase in white blood cells and platelet counts, as well as in bone marrow and splenic megakaryocytes (P<0.05). Additionally, the megakaryocyte number and DNA content were enhanced in PKCδ(-/-) mouse bone marrow after culturing with exogenous thrombopoietin compared with wild-type (P<0.05). Importantly, thrombopoietin-induced signaling was also altered with PKCδ deletion because both extracellular signal-regulated kinase and Akt308 phosphorylation were heightened in PKCδ(-/-) megakaryocytes compared with wild-

SUBMITTER: Kostyak JC 

PROVIDER: S-EPMC4239172 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature