TL1-A can engage death receptor-3 and activate NF-kappa B in endothelial cells.

ABSTRACT:

Background

Death receptors (DRs) play an important role in renal pathology. We have shown that DR3 is inducibly expressed on renal tubular epithelial cells in the setting of inflammatory injuries. In this study we investigate the expression of DR3 in renal endothelial cells and their response to TL1A, the only known ligand of DR3.

Methods

We did RT-PCR, flow cytometry and subcellular immunoblotting to examine the expression and function of DR3 in cells in vitro. We did organ culture of human and mouse tissue to examine expression and signal of DR3 in vivo.

Results

DR3 is expressed in some interstitial vascular endothelial cells (EC) in human kidney in situ; these EC also respond to its ligand TL1A by activating NF-κB. Very low levels of DR3 can be detected on the cell

SUBMITTER: Wang J 

PROVIDER: S-EPMC4239315 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature