Identification of six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay.

ABSTRACT:

Background

Leishmaniases are tropical zoonotic diseases, caused by parasites from the genus Leishmania. New World (NW) species are related to sylvatic cycles although urbanization processes have been reported in some South American Countries such as Colombia. This eco-epidemiological complexity imposes a challenge to the detection of circulating parasite species, not only related to human cases but also infecting vectors and reservoirs. Currently, no harmonized methods have been deployed to discriminate the NW Leishmania species.

Findings

Herein, we conducted a systematic and mechanistic High-Resolution Melting (HRM) assay targeted to HSP70 and ITS1. Specific primers were designed that coupled with a HRM analyses permitted to discriminate six NW Leishmania species. In order

SUBMITTER: Hernandez C 

PROVIDER: S-EPMC4239372 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature